FastQCFastQC Report
Sat 7 Sep 2019
H7TFNBGXC_n01_0822-10R-DREB2A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7TFNBGXC_n01_0822-10R-DREB2A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26800502
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT827730.3088486924610591No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT294320.10981883846802572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA221150.035.9357154
TTGTACT224400.035.1044121
TGTACTC231250.034.5629232
ACTCTAG293450.034.4396865
GTACTCT236250.033.727873
TCTAGTT258500.031.5685277
CTAGTTG254500.031.363578
CTCTAGT260600.030.8586246
ACCTACT226700.027.95016733
GGACACC274700.027.94922329
ACCTCTA290200.027.39663717
CTAATGC290700.027.02924221
GACACCT286900.026.87055230
ACACCTA238950.026.69497531
GTTACCT297450.026.63481114
TAGTTGT305100.026.3225339
TACCTCT304500.026.00660516
ATGCTGG301050.025.80055424
TTACCTC309650.025.55141815
TGGACAC304650.025.29216228
Produced by FastQC (version 0.11.5)