FastQCFastQC Report
Thu 30 Jun 2016
H7T3CAFXX_n01_js_hho5ko_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7T3CAFXX_n01_js_hho5ko_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11460635
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC571450.49861984087269157No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG400690.3496228612114425No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG394150.34391637112603274No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT302880.2642785500105361No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA262410.22896637053706012No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG190600.16630841135766036No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC173980.15180659710391264No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG170980.1491889411014311No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC167690.1463182450187097No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA163170.14237430997497083No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC143860.12552533083899803No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC142320.12418160075772415No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG139300.12154649371522608No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT137770.12021148915396049No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC137650.12010678291386123No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT132750.11583127810980805No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT131590.11481911778884853No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC123710.10794340802233035No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG122390.10679163938123848No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA122340.10674801178119711No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC121320.1058580087403534No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA119350.10413908129872385No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC118270.1031967251378305No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC116890.10199260337668899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT66350.035.409041
CTAGCAG76650.030.408548
ACTCTAG83650.028.5012865
CTGACTC82700.028.0670622
GACTCTA86350.027.6505264
TAGCAGA96550.024.503499
CTCTAGC100850.023.7445226
CTATCGA101550.022.67668217
TCTATCG105200.021.95633316
TGACTCT107500.021.689383
GTGCGCA127250.021.12347868
TGCGCAT130700.020.67264769
ATCTATC113600.020.33280415
GATCTAT114400.020.12942914
GGTGCGC135750.019.87808667
GCAGATC119950.019.60661111
AGATCTA121750.019.08669513
AGGTGCG141250.019.07937866
AGCAGAT126800.018.71302410
TATCGAT126050.018.46342818
Produced by FastQC (version 0.11.2)