FastQCFastQC Report
Thu 30 Jun 2016
H7T3CAFXX_n01_js_hho5ko_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7T3CAFXX_n01_js_hho5ko_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9126427
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC449130.4921203007485843No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG362070.396726999514706No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG302180.33110438510054374No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT184940.20264228268083445No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA168200.18429994564137753No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG150250.16463178854112348No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA144740.15859437652873354No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC144440.15826566081118054No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG135670.14865620466804808No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC123780.1356281050623645No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG117660.12892230442428346No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC117360.12859358870673046No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC115990.1270924535965718No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC108480.11886360346716189No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT103930.11387808175094152No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC103870.11381233860743092No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT102720.11255226169014446No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG99140.10862958746067876No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC98080.10746812525865818No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT97980.1073585533528072No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA95740.10490414266174486No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC94800.10387416674674547No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC94710.10377555203147956No Hit
GGGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCT92580.10144167043685333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT48000.029.760251
CTAGCAG53250.026.6833768
CTGACTC58850.024.8063012
ACTCTAG60150.024.7356515
GACTCTA64200.023.5569324
GTGCGCA97200.021.74776368
TGCGCAT98300.021.46879869
TAGCAGA67700.021.1431189
GGTGCGC105600.020.28296367
CTCTAGC73300.020.15486
CTATCGA73300.019.5277117
AGGTGCG109850.019.33882966
CCGGGGT7100.019.2319761
TCTATCG74550.019.05945216
TGACTCT82550.018.4901083
ATCTATC82950.017.08718915
GATCTAT84050.017.03011714
CGCTTCA191600.016.5674569
GCGCTTC193100.016.40250868
AAGGTGC130750.016.35473365
Produced by FastQC (version 0.11.2)