FastQCFastQC Report
Thu 30 Jun 2016
H7T3CAFXX_n01_js_hho5ko_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7T3CAFXX_n01_js_hho5ko_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10371094
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC476320.45927652376885214No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG379090.36552556557678484No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG331100.31925272300106433No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT214600.20692127561470372No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG168570.16253830116668502No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA161990.15619374388082877No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC158110.1524525763627251No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA155140.14958884761819727No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG141720.13664903625403454No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC128020.12343924372877153No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC121660.11730681449806549No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC121590.11723931920779042No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG121370.11702719115264022No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC113180.10913024219045744No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC113170.10912060000613244No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT111380.10739464901195572No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC109330.10541800122532878No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT106890.10306530825002647No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC106100.1023035756883507No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC105700.10191788831535034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT53550.030.7305851
CTAGCAG65100.025.5892378
CTGACTC67550.024.4104422
GACTCTA73050.023.6747114
ACTCTAG71400.023.1431125
GTGCGCA107250.022.54897568
TGCGCAT111100.021.83036869
GGTGCGC116050.020.89941667
TAGCAGA80950.020.4059379
AGGTGCG119150.020.38513466
CTCTAGC85350.019.6886396
CTATCGA82650.019.52024517
TGACTCT89150.019.202843
TCTATCG87100.018.56312416
GATCTAT93950.017.28416414
AAGGTGC142500.017.16763565
GCAGATC97700.016.94313611
ATCTATC96950.016.89371915
AGATCTA101450.016.28235213
TAGCATG101600.015.95114927
Produced by FastQC (version 0.11.2)