FastQCFastQC Report
Thu 30 Jun 2016
H7T3CAFXX_n01_js_hho5ko_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7T3CAFXX_n01_js_hho5ko_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12376991
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC585930.47340262265683153No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG432640.34955184180064447No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG404600.32689690087033274No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT261800.21152152409256825No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA226950.18336443809323283No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG202220.16338381436974464No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC184870.1493658676814098No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG177090.14308001031914785No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA174060.14063191934130032No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC168040.13576805541831613No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC152450.12317210216925907No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG146900.11868797513062747No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC143050.11557736448220735No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC141180.1140664964529747No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT138350.11177999563868149No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT136310.1101317759704277No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC135260.10928342761176767No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT128040.10345002270745773No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA127790.10324803500301487No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT127150.10273094647964114No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC126990.10260167434879769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT61300.030.611621
CTAGCAG75250.025.3935978
ACTCTAG80200.023.919365
CTGACTC80750.023.6262912
GACTCTA84550.023.0199584
GTGCGCA136300.021.54385668
TGCGCAT140400.021.08888469
GGTGCGC144700.020.38996167
CTCTAGC95650.019.9460376
TAGCAGA97350.019.8085969
CTATCGA96850.019.5855617
AGGTGCG150250.019.52031966
TCTATCG101250.018.63073716
TGACTCT108250.017.818333
GATCTAT110300.017.03864514
ATCTATC115000.016.5248815
AAGGTGC179950.016.454165
TAGCATG116950.016.10193327
AGATCTA119600.015.88924213
CTAGCAT120250.015.83469826
Produced by FastQC (version 0.11.2)