FastQCFastQC Report
Thu 30 Jun 2016
H7T3CAFXX_n01_js_hho5ko_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7T3CAFXX_n01_js_hho5ko_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8187148
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC412790.5041926688023718No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG292230.3569374829916352No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG270930.3309210973100767No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT213250.26046921345503954No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA165250.20184073867969654No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG131570.16070309221233084No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC124190.15168896421562186No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC120460.14713304315495457No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA116110.1418198376284391No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG114330.13964569835552013No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC102170.12479315141243325No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC99900.12202051312618266No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC99880.12199608459502626No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT98460.12026165888292235No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT97260.11879594701353878No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG93450.11414231182824593No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT91280.11149181619777729No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC91200.11139410207315172No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG91110.11128417368294796No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA86350.10547018326772645No Hit
GGGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCT84860.10365025769657518No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC83980.10257540232569387No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC83740.10228225995181717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT46350.032.4063221
CTAGCAG52550.028.3041468
CTGACTC57450.025.9580922
GACTCTA61100.025.6105864
ACTCTAG60050.025.4754375
TAGCAGA68300.021.879689
CTCTAGC72450.021.3085256
GTGCGCA92550.020.91201268
CTATCGA71000.020.89976317
TGCGCAT94750.020.46314269
TGACTCT76500.019.9972633
TCTATCG74800.019.79134216
GGTGCGC99600.019.5723567
GATCTAT79500.018.84139814
AGGTGCG103200.018.72002866
ATCTATC80050.018.44962715
AGATCTA83900.017.93660713
TATCGAT88350.017.11240418
TAGCATG86950.017.0687527
AGCAGAT89400.016.98981710
Produced by FastQC (version 0.11.2)