FastQCFastQC Report
Wed 18 Aug 2021
H7T35DRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7T35DRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002072591
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCGTA742840167.413037405390924No Hit
GGGCAAAT724654847.231560333137582No Hit
AGAAACTC710174137.087052738278119No Hit
TGCTGAGT709947207.0847881318809565No Hit
TCTGTTGG696628886.951880395259709No Hit
CTGCGGCT692798206.9136528253769995No Hit
GAGCACCG676245036.748463495295821No Hit
CCTACGTA609570496.083097127641126No Hit
AACCCGTT596609305.953753304484905No Hit
GACTCAAA570058165.688791062842272No Hit
TCTATCCG504082325.030397243945773No Hit
CCTAACAA462401124.614447337977334No Hit
GGAGGAGG449127634.48198697413529No Hit
ATAGTTAC443993834.43075515673894No Hit
AAATGTGC423508414.226324657551681No Hit
TTGTTTCC380816413.800287658002613No Hit
GGGGGGGG255945972.5541659586216547No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)