FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331385
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37810.1134963386099175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC12550.049.664536145
GCTGCCC12100.043.72493145
CCGACGC38550.034.216743145
CTGCCGC45750.032.00014145
GTATAAG15000.030.4552821
GTGTATA17850.027.2176061
TCTGACC20550.023.629503145
TAGACCG5450.022.612835
TATAAGA28500.021.3685992
CATCTGC33900.019.02751145
TAGGGCC2307.9716503E-418.9114974
GCCGTAA7700.018.824823145
GTATTAG10500.018.646091
TAGGACA12100.018.572864
GGCTTAG24350.018.4631611
GTCCTAG8450.018.0208761
TTACGTT13750.017.921232145
TGTATAA21200.016.7572142
CCCCTAG12150.016.7107181
CCGTATA11400.016.529516145
Produced by FastQC (version 0.11.2)