FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391479
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86690.16079075889936695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC19850.046.73279145
GCCGACC21100.046.025085145
CCGACGC61450.030.30983145
CTGCCGC81850.027.094118145
TCTGACC36850.022.616877145
GTATAAG23350.021.1173821
CTGTACG19000.020.6012239
TTATACT46650.018.0255034
TATACTA20300.017.8544715
GTATTAG21550.017.8339141
GTCCTAG12650.017.7700731
CACTAGG28700.017.4335271
TATACAG31850.017.2972535
CCCCTAG36100.017.0737841
GTGTATA30200.017.0478441
GTATATA35650.016.8824941
TTACGTT25400.016.834126145
CTACACT21150.016.7944874
TAGGGGT38200.016.699384
CCTAAGC64800.016.6705042
Produced by FastQC (version 0.11.2)