FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2598.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2598.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031902
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29700.14616846678629186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7850.048.93986145
CTGTACG10100.031.58299
GCCGACC7950.031.000475145
CCGACGC26450.026.856964145
TATACCG4100.026.5226785
CTGCCGC35200.024.9172145
TAGGACA14600.023.835254
CCTTAAC11600.023.1280613
GTATAAG8750.021.5520761
CTCTAGG17500.021.1376131
GTCCTAG15950.020.9180411
CTTAACT13550.020.3317934
CTAGGAC15450.020.1806663
TTAGGCT41200.020.0603754
ACAGTGC8900.019.5488198
TACACAG11250.019.3320875
CCTAGAG18300.019.0189113
GTTAAGG11550.018.8392281
ACAGCGC2701.08060485E-418.794628
TAAGGGG23550.018.7816813
Produced by FastQC (version 0.11.2)