FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2597.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2597.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074486
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33080.15946118701210807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC8000.038.05232145
GCCGACC8550.033.909058145
GTATAAG11650.031.1225621
CTGCCGC23200.024.36848145
GTCCTAG12250.023.086591
TCTGACC15500.021.510374145
TAGTACC5850.021.0657884
CCCCTAG26700.020.3695411
CCTAGAG15800.019.7285863
ACAGTGC8450.019.7279748
CCTAGTG26000.019.238023
TCCTAGA18100.019.2274022
TAATACT13300.018.5315554
CTAGGGG27250.018.3555413
TATAAGA20550.018.3463422
CCCTAGT27550.018.1587282
CCTCTAT59150.018.1363439
GTGTATA13600.018.1288931
TTAATAC9650.018.0288723
ACAATGC18500.018.021778
Produced by FastQC (version 0.11.2)