FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635945
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89450.24601582257157356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC14650.045.01975145
GCCGACC14100.042.663677145
GTATAAG16750.032.9013061
CCGACGC49950.025.972683145
GTGTATA20600.025.6962661
TAGGCAG5100.025.5846395
GTCCTAG5750.025.2218251
CTGTACG6650.023.9809289
TTACGTT14600.022.83517145
TGTATAA23600.022.425472
GCCGTAA9450.022.241573145
TACCCTA6100.021.3904345
TATAAGA28900.021.3231722
CTGCCGC59700.020.638296145
GTACTAG10050.019.481041
TAGACCG5255.0931703E-1119.3306145
GTGGTCC14300.019.25958145
CCGTATA12250.018.932728145
TTAAGGT13050.018.8862324
TAAGAGA38150.018.8112474
Produced by FastQC (version 0.11.2)