FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133112
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3060.22988160346174652No Hit
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT2360.17729430855219663No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2140.16076687300919526No Hit
GGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAA1990.14949816695714885No Hit
GTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAA1840.13822946090510246No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG1550.11644329587114612No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG300.001931804672.502269
GCTGCCC554.0415525E-665.91114145
TTAGGAC350.003559274862.144798
ACTGCTT400.006038666254.376698
ATACCGT604.928779E-448.334846
TACCCCA450.00961973848.3348355
CCCCACT450.00961973848.3348357
CCTGGTG752.5381094E-548.3348353
CATCCCT450.00961973848.3348355
ACCCCAC450.00961973848.3348356
TCAATAG700.001053177541.429863
CCCCTGG907.4296084E-540.2941741
CCCTGTG2200.039.5466842
CCCTGGT750.001478537838.667872
CCTGTGA2350.037.0224273
GGCTTGA800.00202623336.264751
CTGTGAC2551.8189894E-1234.118714
TTAAGGT1102.4244294E-432.955574
ACAACGT1352.1279082E-532.2232254
CTATTTA1153.1399864E-431.534571