FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402296
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32400.23104965000256722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC6100.043.960476145
GCTGCCC5450.041.224525145
CCGACGC16350.035.46196145
CTGCCGC19050.031.196749145
CCGCGCT1801.5220081E-424.163639
GTATAAG6050.023.9699131
TATAAGG5850.023.545732
ACCGCGC2003.107286E-421.746498
CTGTACG9200.021.2744989
GTATTAT10400.020.9160541
GTGTATA8900.020.3676951
ACCGTGT3651.3634417E-719.859818
CTATAGT5204.3655746E-1119.518174
TTAAGGT4954.7475623E-1019.0393684
GTATACG4202.8550858E-818.990451
CCCCTAG11950.018.8098641
ACAGTCC3902.7380884E-718.5867428
AGGCTTA21550.017.827756
GTATATG7350.017.7573051
GCCTAAG27550.017.6337661
Produced by FastQC (version 0.11.2)