FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040717
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28230.13833373270277063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC9100.046.195015145
GCCGACC9250.040.744606145
CCGACGC25350.029.448812145
CTGCCGC30100.027.691078145
GTATAAG8950.027.5465151
CTGTACG6150.022.3949899
TTACGTT9300.021.042112145
GTGTATA11800.019.664311
GTAGATA14200.019.395624145
GTCCTAG4654.494723E-918.7128121
TACACCG4354.2808097E-818.3319115
TCTGACC13350.017.916006145
CTAAGGT4054.0681334E-717.8998454
CATCTGC25300.017.761501145
GGCTATA3854.9605987E-616.9508911
GTATTAG7001.0913936E-1116.5742051
TACACTG10650.016.3367615
GTATAGA9400.016.199521
CCTAGTG21500.016.1855853
CCCCTAG21550.016.1511971
Produced by FastQC (version 0.11.2)