FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814684
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33370.18388876520650427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC8100.044.733067145
GCCGACC7500.030.919497145
GTATAAG10200.029.1492021
CTGCCGC17100.028.393724145
GTGTATA12350.025.8362031
CCCTGTG7750.025.259992
CTGTACG9950.024.7689299
CTATACT6450.023.6012464
CCCCTAG17650.021.7757991
CCTAGTG17800.021.5857493
CAATGCG13550.021.3979459
CGTTATG10700.020.3320161
CCTAGAG11350.019.8005433
CCTAAGC35600.019.1447182
CCCTAGT20100.019.1183722
GCCTAAG37950.019.1087021
GTTAAGG8350.019.1064131
TAATACT11550.018.8284514
CTAAGCC36750.018.7403433
AAGCCTA38050.018.4801035
Produced by FastQC (version 0.11.2)