FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2588.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2588.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120004
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC37440.17660344037086723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33940.1600940375584197No Hit
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT30280.14282991918883173No Hit
GGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAA25250.11910354886122856No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG23960.11301865468178363No Hit
GTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAA22770.10740545772555145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7100.035.731125145
CCCCTAG76300.029.1725061
CCTAGTG74750.028.9966723
CCCTAGT78200.028.1815742
CAATGCG19550.026.6958149
AGTGACC88950.026.5655526
TAGTGAC88600.026.343255
CCCTGTG21500.026.2999292
CTAGTGA93300.025.0173854
CCTGTGA22300.024.7056873
CGTTATG15750.023.4773751
TCGACCT9900.023.4294455
GTGACCT98050.022.5464927
ACAATGC23250.021.8228788
CGACCTC10800.021.4769926
CTGTGAC26400.021.4169864
CTGCCGC22500.021.26172145
GACAATG29150.020.3892867
TAACGTT17750.020.0099015
GCCGACC8100.019.686777145
Produced by FastQC (version 0.11.2)