FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2587.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2587.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082234
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23700.21899145656115038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGG1203.61797E-948.3392941
GTATAAG4900.042.9134561
GGAGGCC1651.369699E-939.5338868
TATGGTG2451.8189894E-1235.5030985
CCTATAC1656.013579E-835.154233
TAGGACA2154.8748916E-1033.7172974
CTAGCAC1153.1608384E-431.5241723
CTAGTAG1453.4888188E-530.0023143
TAAGACA3400.029.8497244
GTATTAG3001.8189894E-1129.0035781
GTCCTAG1753.7713653E-629.0035781
GGTTAAG2502.4447218E-929.0035781
TAACGTG1255.160158E-428.9941965
TATACCA2502.4519977E-928.9941965
TATACCG1753.7808804E-628.9941945
GTATGGG2553.0213414E-928.4335633
GCTGCCC3850.028.237186145
TTAAGAC3102.7284841E-1128.0666833
CAGGGCA1306.48738E-427.881619
CTATACT2354.3028194E-827.7629664
Produced by FastQC (version 0.11.2)