FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2585.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2585.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705591
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28740.16850464149963268No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC20800.12195186301991509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC7500.039.6174145
GCCGACC6700.032.449635145
ACAACGT12400.028.6418764
CAACGTT12700.028.5335065
CTGCCGC15700.028.157465145
CACAACG12700.027.3969863
CCCCTAG28900.027.3485341
CCCTGTG11000.027.0213722
CAATGCG17600.025.5332499
GTATAAG9500.024.4248431
CCTAGTG27750.024.2932263
CGTTATG15350.023.6192971
CCCTAGT29850.023.5582832
ACAATGC20200.022.962418
CCCTAGA5200.022.3065722
ACGTTTC26300.021.7694517
CTGTACG7400.021.5485489
CCTGTGA13950.020.785053
CGGTGTG9900.020.4980095
TCACAAC18500.020.3773562
Produced by FastQC (version 0.11.2)