FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2584.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2584.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972587
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35860.1817917283242767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC6900.032.56459145
GCTGCCC6550.032.09148145
CCCTGTG11900.030.4586852
CAATGCG18400.027.5776549
ACAATGC22750.025.4902828
CCTAGTG40150.024.915563
CCCCTAG43050.024.0833591
CCTGTGA15050.023.601343
CACAACG11700.023.5437053
CTGTACG14550.023.4159589
CGTTATG15650.023.1638031
CCCTAGT44150.022.9871652
TCGACCT8850.022.9340575
CGACCTC9500.022.1279146
ACAACGT13500.022.015434
AGTGACC53100.021.0228826
CTGCCGC16900.021.01563145
GACAATG27350.020.93757
CTAGTGA53900.020.8458714
TAGTGAC52850.020.7108575
Produced by FastQC (version 0.11.2)