FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240399
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5400.044.286636145
GCCGACC5800.027.488258145
CTGCCGC11550.026.352377145
CTGTACG6400.024.9172619
CCTAGTG13650.023.3731753
CCTTAAC5900.023.3506663
GTCCTAG6250.023.2088051
TACCGTG3452.9740477E-923.1107277
GTATAAG5700.022.9034271
CCCCTAG14100.022.6327021
CTTAACT7050.022.6217484
TAGGACA6700.021.6395284
GTGTATA7900.021.1156061
CCTGGAC3457.497147E-821.017383
CTCTAGG7650.020.8575861
CCCTAGT15050.020.7188032
CTAGGAC6400.020.3934273
GCCTAAG20750.019.9232221
TCTAGGA9900.019.777042
TAAGGGG12400.019.2970073
Produced by FastQC (version 0.11.2)