FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2582.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2582.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574804
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5980.10403546252287735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC2900.057.484203145
GCCGACC1900.053.406376145
ACCCTCG850.002743544834.111247
CTGCCGC4900.031.06289145
GCAGCGT1000.00607791128.9945538
TATGTCG1358.087375E-426.846815
CATCTGC5750.026.470985145
CCTCTAT14400.026.175649
CTGTACG2151.8666573E-523.6002189
ACCTCTA16450.022.9136288
CTAGGAG1600.002176407322.6519933
GTGTATA3604.838512E-922.1524731
CCTAGTG5300.021.882683
CCCCCCA1700.003091459921.3232351
ACCGTGT2053.6684723E-421.2155278
CTATTTG17700.020.8895421
GTATAAG2857.1008417E-620.3470553
TTAATAC3701.5700243E-719.5909143
TATACCC2256.8714947E-419.3297024
ATCGTAG1900.005885374719.0786841
Produced by FastQC (version 0.11.2)