FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168378
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41240.1901882420869424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC9850.050.77436145
GCCGACC9050.048.054527145
CTGTACG11600.024.9956059
GTTAAGG12900.024.166881
GTGTATA14800.023.5137211
CTGCCGC24900.022.996376145
GTATAGG6150.021.2196981
ACCGGCC3601.1798329E-720.1353478
GTCCTAG6350.019.409621
CCTTATA7900.019.2705442
GTATAAG12050.019.251063
TCTGACC14700.019.229984145
GTATTAG9050.019.2266881
CCCTAGG2659.3554656E-519.1511121
CCCCTAG27550.018.9475351
TCTATAC11150.018.8544923
CCTAGCC2701.0807921E-418.7942923
CCTAGTG26350.018.7076843
CCCTAGT29100.018.1857642
CCGTACG2400.001061505418.1218159
Produced by FastQC (version 0.11.2)