FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681500
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24060.14308652988403212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC8450.040.310883145
GCTGCCC7350.036.48342145
CTAGGAC12000.027.7932013
CTGTACG7950.027.353499
TAGGACA12150.026.8525354
GTCCTAG11550.026.3697991
CTGCCGC28500.024.920822145
CCGACGC19100.024.663866145
CTCTAGG12850.023.1377031
CCCCTAG18050.022.4983331
CTACACT7150.022.308264
CCTAGTG18350.021.7314453
TCTAGGA15850.021.500252
GTATAAG5900.020.8947121
CCCTAGT19600.020.7160452
TAGCACC3157.286053E-720.7148134
CCTAGAG13500.020.4085333
TACACCG3208.4780913E-720.3905375
TCCTAGA17150.020.2932682
TAGGACT5750.020.1744254
Produced by FastQC (version 0.11.2)