FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2579.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2579.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435413
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6500.047.950924145
GCCGACC6500.040.14496145
GTATAAG7500.029.0016651
CTGCCGC15150.028.228075145
TATACCG4053.274181E-1123.2656445
GTTACGC2202.2340993E-523.0622738
TACCTCG2903.2962453E-722.4942367
GTCTTAG3004.5552588E-721.751251
GTGTATA9450.021.4827181
TCTGACC10350.020.309513145
GTCCTAG8250.020.2132821
GTACTAC3651.3603494E-719.8641551
GTATAAA13600.019.7253991
CTAGGAC9250.019.5936723
TAGGACA9750.019.3304024
ACCTGCG3001.10852E-519.328387
ACCGTGT3751.8144237E-719.328388
CACTAGG19550.019.2849961
GGGTATG5356.548362E-1118.9730511
CCCCTAG18400.018.914131
Produced by FastQC (version 0.11.2)