FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2578.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2578.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561426
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18850.12072298014763427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5800.043.73387145
GCCGACC5500.036.89548145
CTGCCGC14600.030.77632145
GTATAAG9050.027.2371231
CTGTACG6850.024.3387689
GTGTATA10950.023.8352261
ACAGTGC5300.021.8822278
CTAAGCG2003.1064774E-421.7475553
ACAATGC13350.021.7183158
GTATTAT12800.021.5230871
CAATGCG10400.020.909779
CATCTGC15800.020.641123145
GTTAAGG10250.020.511861
CTCTAAG4601.7826096E-1020.4887961
CTAGGGT2505.9853744E-520.2964154
CGTTATG7750.019.644841
CCCCTAG13400.019.4772931
GTCCTAC2659.3551316E-519.150631
TTATACT16400.019.005794
CCTATAG3902.734414E-718.589441
Produced by FastQC (version 0.11.2)