FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2577.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2577.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917697
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43090.22469660222652482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC7350.049.30425145
GCTGCCC7900.043.119377145
CCGACGC23800.029.234522145
CTGCCGC32300.024.45827145
GTGTATA14150.024.0856481
GTATAAG10000.023.2042181
TACCGTG5100.022.7421117
TAGGACA7250.021.9976844
TATACTG13150.021.4985165
TCTGACC13400.021.094124145
CTGTACG6850.020.1084239
TACACTG10900.019.9509895
TTATACT17550.019.4139144
CCCCTAG21300.019.4049341
TATACCG5707.2759576E-1219.0759455
GTATACG5356.548362E-1118.975411
CCTAGTG19550.018.912043
CTAGGAC7050.018.5096573
GTATTAG8300.018.3467061
CCCTAGT22100.018.043442
Produced by FastQC (version 0.11.2)