FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145694
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2500.17159251582082996No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAC100.007066281145.126161
GCGACTC100.0070880977144.976658
AGGACCG300.001933645972.488335
TACTGGG400.006044398554.3662457
TACACAG400.006044398554.3662455
CCTTGCA450.00961579148.342159
TACCCTA450.00962885548.325555
TATATGG604.934998E-448.325552
TAAGGTA450.00962885548.325555
TAGGTTG657.321676E-444.60825
CCTTTAA907.4128046E-540.312821
TCTGACC1105.2515243E-639.525513145
ATTTACC750.001480393938.660443
TGGACTA750.001480393938.660445
AACAGCC850.00273649434.1121527
CCGACGC1956.9394446E-933.444668145
TCTGTAC900.003620991332.2170333
CTAATAT900.003620991332.2170333
ACCTTTA1601.8649316E-631.7136428
TATGACA1402.740156E-531.0557614
Produced by FastQC (version 0.11.2)