FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888449
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37710.19968768020740832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC8050.051.31486145
GCCGACC8700.033.319996145
CTGCCGC21800.027.9246145
CTGTACG6300.025.3140359
GTATAAG9400.022.3669573
CCCCTAG20300.021.0763931
CCTAGTG20000.021.0249393
CCCTAGT21900.020.195612
CCGTGCG1800.00431184320.1361629
GTGTATA13900.019.8248121
GTCCTAG9450.019.1844141
CTTAGGC45050.019.1508873
TTAGGCT47850.018.6353234
TGTATAA15650.018.5317692
CCCTTAC5107.057679E-1018.4847451
CCTCTAT45450.018.3418529
AGGCTTA45450.018.1818776
GGCTTAG45950.018.1489961
GCTTAGG45300.018.0863742
TAGGCTT47850.018.0278635
Produced by FastQC (version 0.11.2)