FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2574.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2574.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865181
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43090.23102315539349802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5700.043.23443145
GCCGACC5900.035.626373145
GTATAAG9200.025.217631
GCCGTAA3301.7807906E-924.160416145
CTGCCGC19600.024.037148145
GTCCTAG11500.019.5436631
AGGCTTA45300.018.5633056
CCCCTAG18700.018.2220951
TAGGCTT47200.017.9696395
TAGGACA11700.017.9683284
GCTTAGG45750.017.9029528
CTTAGGC46500.017.7719783
TTAGGCT49050.017.7352684
CTGTACG6950.017.7301759
TCTGACC13500.017.717638145
GTTAAGG9100.017.5276371
CTTAGCC6253.274181E-1117.3978313
GGCTTAG46950.017.2951561
ACAGTGC7600.017.167078
ATAATAC16900.017.1576233
Produced by FastQC (version 0.11.2)