FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2573.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2573.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352465
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54210.23043913511996988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC10750.044.500317145
GCTGCCC9300.037.409847145
CCGACGC25600.034.825134145
CTGTACG15450.024.3982079
CTGCCGC31150.023.966621145
GTATAAG12500.022.6248341
TCTGACC16750.022.501741145
GTGTATA15650.022.2411731
CCTTAAC15000.022.2328833
GTCCTAG14300.020.2840541
TGTATAA16650.019.5962812
GTCCTAA5759.094947E-1218.9170861
CCTAGAG17250.018.912663
GTATAAA19700.018.7730461
GTATAGG7400.018.6188431
TACACCG5509.822543E-1118.4526025
GTATATA18500.018.030881
TTACGTT8850.018.01802145
CCCCTAG30250.017.7393281
CTACACT8050.017.1103634
Produced by FastQC (version 0.11.2)