FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115797
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39030.12526490012025818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGC34850.034.31519145
GCCGACC11850.033.027878145
GCTGCCC10150.026.420494145
GTCCTAG6400.024.924661
GTATAAG15600.023.2397751
CTGTACG14050.020.1209879
GTGTATA19950.019.9897021
GTATTAG12800.019.2599641
CCCCTAG36950.018.249671
CATCTGC33350.017.82064145
TACCCCA11150.017.5523785
CATACCC11800.017.2014453
TATACCG8850.017.199795
CCCTAGT37350.017.08112
CTAGGAC7700.016.9461363
GTTAAGG19150.016.6598241
CAATGCG19050.016.3619299
TATAAGA27050.016.3488182
CTATTTG90000.016.274041
GTATTAA11650.016.1820641
Produced by FastQC (version 0.11.2)