FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2570.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2570.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506839
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20740.13763912402054898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4700.032.378136145
CTGCCGC13200.030.1939145
GTATAAG8900.026.8888071
GTGTATA10950.025.1661661
ATTACCC4752.728484E-1019.8418433
GCCGACC4954.783942E-1019.031305145
CTAGGAC5056.202754E-1018.6631183
CCTAGTG15050.018.3054053
CTACCCT2801.4282856E-418.1223534
CCCCTAG17150.017.7595831
CTTAAGG2500.001393962317.3997693
TGTATAA11700.017.3513492
TATAAGA14100.016.9689872
GTATTAT12050.016.8507251
GTATAAT13850.016.755131
TACCCTT5201.7997081E-816.7272155
CCCTAGT17850.016.6535452
TTATACT13550.016.5842934
CTAGGGG16650.016.5463283
CACTAGG15350.016.5351181
Produced by FastQC (version 0.11.2)