FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683684
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC22550.1339324956464515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22080.13114099795448553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6950.036.49804145
CTGCCGC13900.032.848236145
GCCGACC5950.030.451548145
CCCCTAG45250.024.838861
CTGTACG6200.024.5551689
CACAACG11000.024.3843653
CCTAGTG39300.024.349153
CCCTAGT46200.023.539832
ACAACGT12450.023.2912644
CAATGCG15100.022.084949
TAGTGAC52800.021.6939415
ACAATGC17450.021.6028148
AGTGACC55250.021.256816
CAACGTT12750.021.0381165
CTAGTGA54650.020.958944
CGTTATG11850.020.8055461
CCCTGTG11500.018.9137422
GTGTATA12400.018.7131481
GTATAAA17250.018.4961831
GTGACCT60850.017.9894877
Produced by FastQC (version 0.11.2)