FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2568.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2568.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913811
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57100.19596329343255278No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC44120.15141682147538052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC10800.038.25409145
GCCGACC11500.032.77421145
CCCCTAG49800.026.3526971
CCTAGTG47750.026.2643453
CTGCCGC34500.025.631113145
CAATGCG28400.025.5246969
CCCTAGT52100.024.7680052
CGTTATG23000.023.328121
ACGTTTC41300.023.1671717
ACAATGC31850.022.9866668
CACAACG18450.022.3960973
CCCTGTG20350.022.0869662
TAGTGAC60200.022.035665
CTAGTGA61000.021.7470424
AGTGACC62500.021.5723256
TAACGTT26950.021.518045
CAACGTT20550.021.164645
ACAACGT20450.020.2050744
GACAATG39650.019.9265657
ATAACGT28550.019.5507554
Produced by FastQC (version 0.11.2)