FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2567.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2567.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20356
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280.1375515818431912No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC260.12772646885439182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAG100.0070556197144.894351
ATCCCCA100.0070556197144.894356
CTCGATC100.0070556197144.894357
TATAGTT100.0070556197144.894359
CGCTTTA100.0070556197144.894356
TTCTCTA350.003526685562.097585
AAAGGGG2000.00395463910.867077145
AAAAAGG2701.9568666E-410.732915145
CATTAAA2950.00403209877.3675094145
AAAAGGG2403.9025137E-47.244717140-144
GGGGGGG48700.06.991822145
AAAAAAG3552.4224391E-66.9386024140-144
TATCATT3151.8156672E-46.899731145
ATCATTA2957.710284E-56.8763423130-134
CGCCGTA3501.4661762E-56.6237416140-144
TCATTAA2800.00224896786.209758130-134
GCCGTAT3507.056527E-46.209758145
GTGGTCG4152.7368624E-55.935431135-139
TAAAAAA4051.2316968E-45.724221135-139
TTAAAAA3600.00101033735.6347804135-139
Produced by FastQC (version 0.11.2)