FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833646
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44230.2412134076043031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC6950.037.535946145
GCTGCCC8100.035.785294145
CTGCCGC23600.032.547943145
GTATAAG10250.026.1781711
GTGTATA12950.025.7602161
GCCTTAG2802.3384018E-723.3101941
TGTATAA15850.020.126962
ACCGTGC2959.58421E-619.6574848
CCTCTAT37600.019.2789769
TATACTG12800.019.2538265
TATACCG4705.155016E-918.5068075
GGACTTA3151.6881757E-518.4083866
GACCGTG3151.6885724E-518.4078857
TATAAGA16100.018.0131172
CTAGGAC5251.0404619E-917.9520953
CCTTACA5351.3369572E-917.6175022
TTAGGCT47950.017.5373884
CCCCTAG26000.017.2933751
AGGCTTA46150.017.11956
CTTAGGC45250.016.9831523
Produced by FastQC (version 0.11.2)