FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105097
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22010.10455575206273154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGC20600.035.530087145
GCTGCCC8200.029.16367145
GCCGACC6800.028.77378145
GTATAAG12400.028.6560151
GTGTATA15350.025.9833831
GTATTAG9400.020.8294261
CCCCTAG27750.020.6445541
CCTAGTG27150.020.5602363
CCCTAGT28400.019.9143452
TCTGACC14350.019.694946145
CTGTGCG3502.0062162E-618.6379899
TGTATAA18450.018.0780352
TAAGCCT57250.017.9786934
CTTAGGC39950.017.9649333
AGGCTTA40950.017.877326
TATAAGA21000.017.6092412
TAGGCTT44150.017.5666125
GTATATG10850.017.3774091
TTAGGCT45050.017.2160784
CTAAGAC5501.9354047E-917.1351473
Produced by FastQC (version 0.11.2)