FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411265
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21410.15170786492969074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGC13750.031.620632145
GCTGCCC4750.027.460022145
GTGTATA9050.025.6362361
GTCCTAG6100.023.7713051
GGGCCAC1851.8342824E-423.5076526
GTATAAG8000.021.7507461
TAGACCG2704.455249E-621.4768895
CTGTACG5450.021.2790979
GCCGACC4551.546141E-1020.703985145
CTTAAGG2155.06243E-420.2296643
CACCGTG2608.098011E-519.5136727
CCTAGAG8350.019.0990663
TGTATAA10500.018.640852
CTAAGCC13500.018.2566493
CCTAAGC13200.018.123052
GTGGGAC2801.4281702E-418.1224083
CTAGAAC11300.017.9620323
GCCTAAG14950.017.9437581
TCTAAGC4054.0687337E-717.8986763
GTTAAGG6256.002665E-1016.2405551
Produced by FastQC (version 0.11.2)