FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650728
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28190.17077313767016733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6250.039.43017145
GCCGACC6500.034.568306145
GTGTATA11800.028.882171
GTCCTAC2903.947207E-1027.504811
GTATAAG9500.026.7095323
CTGTACG6950.025.034289
TGTATAA13800.023.1185862
CTGCCGC16650.022.201674145
TGGACCG3951.4151738E-820.1878995
GTATTAT12450.019.802661
CCCGTAC1850.00504932619.5938663
TACACCG4452.6120688E-919.5486495
CCTTATA5759.094947E-1218.9152052
CTTAGCC6301.8189894E-1218.4120143
TTATACT14350.018.1869264
CCCTGTG7650.018.0053229
GTATTAA8200.017.686021
CCGTGCG2050.00913239917.6817329
TATAAGA15800.017.4355324
ACCTTAG3753.859479E-617.4030441
Produced by FastQC (version 0.11.2)