FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754254
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15700.20815269126845865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3350.062.73187145
GCTGCCC3250.044.594532145
CCGACGC8750.034.783737145
CCATGCA2051.139415E-831.8249449
CTGCCGC10500.027.606142145
GTATAAG3700.027.4305273
CTAGACT4200.025.8893414
GTATAAC2051.2877599E-524.7609461
GTGTATA4850.023.922211
TCTATAC3352.1100277E-923.8043163
GTCTAAC1902.1895669E-422.8992211
GGTGTCG2953.8817961E-722.1127058
GGCTAAG2653.77146E-621.8910791
CTACTAG2104.3070776E-420.7183441
TATACGG1750.003659380120.7128472
AGGTGCC1750.0036650120.7073527
ATTGCGC1800.00431280320.1334828
GTACTAG3259.80468E-720.0808541
CTTAGGC8200.019.4498673
CCTCTAT18300.019.409939
Produced by FastQC (version 0.11.2)