FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6127
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400.6528480496164518No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC100.006920232145.08
AAGGGGG1450.00171921989.0125-129
GGGGGGG38450.07.165149145
CATTAAA2500.00447256776.38120-124
CGTATCA2700.0099511065.9074078115-119
AAAAAAA8851.8189894E-125.734463140-144
Produced by FastQC (version 0.11.2)