FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396089
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23320.16703806132703575No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC16050.1149640173370036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC4350.041.65745145
CGGTGTG8800.040.361845
TGTCGGT10800.031.54962
TCGGTGT10950.031.1129574
GTCGGTG10750.031.0197323
CCCCTAG32350.028.2408831
CCTAGTG30550.028.237383
CCCTAGT33200.026.4220832
GTGTCGG13250.026.2668131
CTGCCGC12350.024.65042145
CGTTATG8400.024.1691151
GCTGCCC4800.024.161324145
GTATAAG8600.023.6070441
TAACGTT11350.022.9913355
CTAGTGA45850.022.9237794
TAGTGAC44350.022.718455
AGTGACC44750.022.515386
CAATGCG11950.022.443549
CCCTGTG10450.022.2000242
ATAACGT11450.020.8913254
Produced by FastQC (version 0.11.2)