FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051716
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19350.18398503017924991No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4050.059.058926145
GCCGACC2901.2732926E-1129.992306145
CTGCCGC10000.029.717377145
GTATAAG5900.029.493781
CCCTTAG1907.1516424E-626.7125681
GTATATG5200.022.3093971
GTATTAG3851.0504664E-820.7158681
TTACGGC1800.004310054220.1365973
CCTGGAC3301.1393349E-619.7704773
TCTAAGG2608.084884E-519.5170083
GCCTGTG2307.9735083E-418.9090927
GTCCAGG2701.07860425E-418.7977311
GGCTTAG12700.018.26397
CAGTGCG2000.00792370818.1220769
TATACCA5301.1823431E-917.780155
GCTTAGG12700.017.6956772
CCATAGG2050.00913019117.6817552
TGCTATG3302.513521E-517.5745942
GTATAGT4557.13535E-817.5288121
GTGTATA7450.017.5181181
Produced by FastQC (version 0.11.2)