FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441890
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26530.18399461817475674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC5350.031.158333145
GTATAAG8000.028.1023751
GCCGACC5300.027.349808145
TAAGGCG2259.129162E-725.7722785
CTGCCGC13900.024.506607145
CCTGCGG1550.001811487523.3812968
CCCCTAG18850.020.3908751
CAGACCG2857.1146515E-620.3465355
CCCTAGA4452.6011548E-919.5538522
CTAGGAC4452.6047928E-919.5524943
CTGTACG5605.456968E-1219.41559
GTATTAG5657.2759576E-1219.2536961
GTGTATA10900.018.6295741
GTATTAA7900.018.3600671
GCCCTAG2400.00105783718.1305661
CTGCGCG2000.007927475518.1211329
AGGCTTA40100.018.0753156
CCCTAGT19950.017.8100132
GGCTTAG40500.017.7276651
CTTAGGC41450.017.6676083
Produced by FastQC (version 0.11.2)