FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842054
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31090.16877898259225843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC6850.037.03116145
GCCGACC6900.035.712452145
CTGCCGC20650.032.640194145
GTATAAG7950.027.3610421
GTTAAGG10000.021.7520281
CCCCTAG27100.020.3341
CCCTAGT27400.019.579992
TAAGGGG15600.018.5899643
CCTCTAT46300.018.4755339
CCTAGTG29300.018.3107953
TATAAGA16100.018.0121442
CTATAGG3652.9851053E-617.878381
CTTAGGC41800.017.6915973
TTAGGCT43150.017.6416764
TTATACT16450.017.6289084
GTATTAG7000.017.6087841
GTATATA13250.017.5110661
TAGGCTT45550.017.5074885
AGGGGTA18750.017.399265
GGCTTAG40950.017.3482767
Produced by FastQC (version 0.11.2)