FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407361
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21150.15028127111665024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC4850.031.383314145
GCTGCCC4200.031.063076145
TATACTA5500.027.6792955
GTGTATA10250.024.761761
GTATAAG7750.024.3212263
CTATACT5550.023.511374
CTGCCGC15150.023.442541145
TGTATAA11950.022.4472482
TAGGACA8900.021.1778454
CCTAGAG8800.020.5954443
GTCTATA6000.020.5463661
GTCCTAG7500.020.3046441
CTAAGGT2505.9809074E-520.298154
CTGTACG5052.910383E-1120.097899
TAGGTTG5900.019.6592245
CCGTGCA2608.083747E-519.5181459
CCTATAC4553.43789E-919.1198163
CCCCTAC3802.0751395E-719.0833131
CTAGGAC8050.018.9119933
CTATTTG38750.018.7139591
Produced by FastQC (version 0.11.2)