FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848114
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59450.2087346222798666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC10100.040.185806145
GCTGCCC9350.037.20794145
CCCCTAG52650.027.1328261
GTATAAG14100.026.7430781
CCTAGTG49250.026.3491863
CGTTATG19550.025.5934891
CAATGCG25300.025.500239
CCCTAGT53500.024.5296272
CCCTGTG18650.023.7146742
GTATTAG11450.023.432761
ACAATGC30500.023.0536088
CTGCCGC28250.021.037851145
CCTGTGA22600.020.2093583
TAGTGAC76100.020.1934535
GTATTAA13300.020.1733151
AGTGACC76850.019.8073866
CTAGTGA77950.019.7148914
GACAATG38850.019.4048067
CTGTGAC25650.019.2174684
GTGTATA16900.017.592371
Produced by FastQC (version 0.11.2)