FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167547
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15020.12864578470930935No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13920.11922432244697644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC4150.045.395027145
CTGCCGC11300.025.007439145
CCCTGTG7900.024.7809182
CCCCTAG20150.024.4720551
CCGTGCG1801.5234637E-424.1597259
GCCGACC3952.3646862E-1123.846756145
CCCTAGT20050.023.5060562
CCTAGTG18200.023.5020393
CCTGTGA8900.022.8083023
GTCCTAT2303.1370986E-522.0702271
TAGTGAC23700.022.0171055
ACGTTTC19500.021.1825927
ACAACGT9050.020.8245324
AGTGACC25300.020.6247186
CAATGCG11950.020.621699
CTGTGAC9500.020.601114
CTAGTGA25950.020.1115534
CAACGTT10450.019.4185945
CTGTACG6500.018.9560939
TGTGACC11950.018.800525
Produced by FastQC (version 0.11.2)