FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452920
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57520.23449602922231463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG11800.030.110541
CTGCCGC27750.024.812483145
GCTGCCC11950.023.654095145
CTAGGAC13150.019.8460883
TCTGACC14600.018.864285145
TAGGACA14500.017.9976164
GTGTATA15450.017.8344441
CTCTAGG14900.017.5194591
CCCCTAG34450.017.4700161
GCCGACC11250.017.394857145
TAAGCCT46150.017.121244
GCCTAAG45200.017.0048141
CCTCTAT54200.016.986459
TTAGGCT51400.016.9238284
CCTAAGC43100.016.8218612
CCCTAGT35050.016.7551482
CTAAGCC44600.016.5791973
CCTAGTG34150.016.557783
CTTAGGC47050.016.332193
TTATACT20000.016.3103394
Produced by FastQC (version 0.11.2)